蜘蛛侠

巴顿·奥斯瓦尔特

饰 Uncle Ben (voice)

吉姆·库宁斯

饰 Hammerhead (voice)

纳吉·杰特

饰 Security Robot (voice) / Miles Morales (voice)

Alastair Duncan

饰 Adrian Toomes (voice) / Thug #3 (voice)

罗比·戴蒙德

饰 Cop #1 (voice) / Peter Parker / Spider-Man (voice) / Bully #1 / Hot Dog Announcer / Ticket Taker (voice)

约翰·迪马乔

饰 Raymond Warren (voice) / Security Guard/Customer (voice) / Policeman (voice)

Nancy Linari

饰 Woman Bystander (voice) / Aunt May (voice) / Landlady (voice) / Bomb Voice (voice)

马克斯·迈特尔曼

饰 Thug #2 / Little Kid (voice) / Police Officer #1/Osborn Technician #2/Police Officer #2 (voice) / Male Clerk/Guy in Line (voice) / Harry Osborn (voice)

梅兰妮·米尼奇诺

饰 Student #1 (voice) / Helicopter Pilot (voice) / Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice)

本·迪斯金

饰 Police Officer #2/Hoodlum #1/Police Officer #4 (voice) / Osborn Recruiter #1 (voice) / Student #2 (voice) / Flash Thompson (voice) / Flash Thompson

史考特·曼维尔

饰 Grady Scraps (voice) / Doc Ock / Otto Octavius (voice) / The Living Brain / Cop #2 (voice)

乔希·基顿

饰 Prison Guard (voice) / Unidentified Voice / Police Officer (voice) / Hologram (voice) / Norman Osborn (voice)

弗莱德·塔他斯西奥

饰 Interviewer (voice) / Trucker/Mr. Slott (voice) / Max Modell (voice) / Bystander (voice) / Thug #1 / Father (voice) / Police Officer #1 (voice)

劳拉·贝利

饰 Osborn Recruiter #2 (voice) / Tully (voice) / Gwen Stacy (voice)

杰森·斯皮萨克

饰 Alistair Smythe (voice) / Alistair Smythe / Scorpion (voice) / Audience Member (voice) / Scorpion / Purse Snatcher / Driver (voice)

亚伦·艾布拉姆斯

饰 Tinkerer / Police Officer (voice) / Tinkerer (voice) / Tinkerer / Old Man (voice)

索菲亚·卡尔森

饰 Sand Girl (voice) / Keemia (voice)

尤里·洛温塔尔

饰 Clayton Cole / Clash (voice) / Curt Connors (voice) / Nocturnal (voice)

鲍勃·裘斯

饰 J. Jonah Jameson (voice)

泽诺·罗宾逊

饰 Randy Robertson (voice)

马修·默瑟

饰 Aleksei Sytsevich / Rhino / Reporter (voice) / Aleksei Sytsevich/Comic Book Guy (voice)

格蕾·德里斯勒

饰 Black Cat (voice) / Black Cat/Nurse/Mom (voice)

卡梅隆·博伊斯

饰 Herman Schultz / Shocker (voice)

Nathaniel J. Potvin

饰 Prowler (voice)

理查德·多伊尔

饰 Police Officer #3 (voice)

丹尼尔·哈根

饰 Cop (voice)

特拉维斯·威林厄姆

饰 Sandman / Flint Marko (voice)

杰里米·沙达

饰 Ross Caliban (voice)

素玛立·蒙塔诺

饰 Yuri Watanabe (voice)

菲利希娅·戴

饰 Mary Jane (voice)

艾利克斯·迪塞尔

饰 Swarm (voice)

伊薇特·尼科尔·布朗

饰 Head Administrator (voice)